Matthijs van Miltenburg is been serving as a Member of European Parliament since 2014, affiliated with the Alliance of Liberals and Democrats for Europe (ALDE) and part of the Dutch delegation of D66. He is a full member of the committee for Regional Development (REGI) and a substitute in the committee for Transport and Tourism and Internal Market and Consumer Protection (IMCO). Within the committee for Regional Development, Matthijs van Miltenburg serves as coordinator for the ALDE delegation within that committee. Finally, Matthijs van Miltenburg is a member of the delegations for European-Latin American affairs.

Previously, Matthijs van Miltenburg has worked at the Brabant Investment Agency as project manager foreign investments, and has gained international experience at the North-Brabant region and the ministry of Transport and Water. Furthermore, he has served as a local councillor for D66 in the city of Den Bosch.